Difference between revisions of "Part:BBa K3790154"

Revision as of 18:25, 21 October 2021

Bst-(G2S)3-Sso7d

Introduction

The fusion protein obtained by linking the C-terminus of Bst to the N-terminal of Sso7d via a (G2S)3 linker.

Experimental Results

The length of Bst-(G2S)3-Sso7d DNA was 1968 bp, which is approximately 1980 bp after adding homology arms to both ends for PCR cloning. We isolated the target DNA by gel extraction for subsequent reactions.

Figure 1. PCR assembled Bst fusions. The first lane was loaded with DNA ladder, sizes were marked on the image. The brightest band of 750 bp was about 100 ng, and other bands about 50 ng. Lanes with correct sized amplified DNA were labeled. The second lane (lane-1) was loaded with Bst-(G2S)3-Sso7d DNA.

After ClonExpress homologous recombination reaction, Fast-T1 competent bacteria were transformed with reactants, and spread onto LB plates with antibiotics. We picked colony after 16 h culturing plates at 37℃. We further grow clones in 2 ml LB liquid medium with antibiotics, 37℃ shaking overnight.

Mini-prep was performed the following day, and the resulting plasmids were sent to Sanger sequencing.

Reference

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 19: / Line 19: @@
 Mini-prep was performed the following day, and the resulting plasmids were sent to Sanger sequencing.
-===Usage and Biology===
-===Experimental Results===
-The length of Bst-(G2S)3-Sso7d DNA was 1968 bp, which is approximately 1980 bp after adding homology arms to both ends for PCR cloning. We isolated the target DNA by gel extraction for subsequent reactions.
-[[File:T--Fudan--bst-6.jpg|600px|thumb|none|'''Figure 1. PCR assembled Bst fusions.''' The first lane was loaded with DNA ladder, sizes were marked on the image. The brightest band of 750 bp was about 100 ng, and other bands about 50 ng. Lanes with correct sized amplified DNA were labeled. The ninth lane (lane-6) was loaded with Bst-(G2S)3-Sso7d DNA.]]
-After ClonExpress homologous recombination reaction, Fast-T1 competent bacteria were transformed with reactants, and spread onto LB plates with antibiotics. We picked colony after 16 h culturing plates at 37℃. We further grow clones in 2 ml LB liquid medium with antibiotics, 37℃ shaking overnight.
-Mini-prep was performed the following day, and the resulting plasmids were sent to Sanger sequencing.
 ===Reference===