Difference between revisions of "Part:BBa K3794001"
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| − | This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein, <a href="parts.igem.org/Part:BBa_K3794000">BBa_K3794000</a>, in E.coli. It is under the control of the <i> lac </i> promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021. | + | <p>This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein, <a href="parts.igem.org/Part:BBa_K3794000">BBa_K3794000</a>, in E.coli. It is under the control of the <i> lac </i> promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021. </p> |
| − | Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column. | + | <p>Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column.</p> |
| − | This composite part / translational unit does not contain a terminator, therefore a plasmid, such as pSB1A3, containing a terminator region or an addition terminator part from the iGEM registry is required. | + | <p>This composite part / translational unit does not contain a terminator, therefore a plasmid, such as pSB1A3, containing a terminator region or an addition terminator part from the iGEM registry is required. </p> |
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Revision as of 18:16, 21 October 2021
PVFP-5 Composite
This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein, BBa_K3794000, in E.coli. It is under the control of the lac promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021.
Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column.
This composite part / translational unit does not contain a terminator, therefore a plasmid, such as pSB1A3, containing a terminator region or an addition terminator part from the iGEM registry is required.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]