Difference between revisions of "Part:BBa K3739020"
(→1. Identification) |
(→Characterization) |
||
| Line 12: | Line 12: | ||
After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1. | After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1. | ||
| − | ::'''Fig. 1.''' Gene circuit involved in protein expression and AGE analysis of string a, Gene circuit was constructed by | + | ::'''Fig. 1.''' Gene circuit involved in protein expression and AGE analysis of string a, Gene circuit was constructed by BBa_K3739020 to express LCIKR-2-GFP. b, Target bands of LCIKR-2-GFP (black arrow, 1200 bp). |
Revision as of 18:13, 21 October 2021
LCIKR2-GFP
LCI-KR2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly. We use BBa_K3739050 to verify its capability by purification the protein.
Biology
LCI-KR2 is a mutant of a peptide called LCI from Bacillus subtilis, can sticks to polypropylene strongly.. GFP is green fluorescent protein from jellyfish Aequorea Victoria, which has been widely used as reporter for decades. GFP is fused at C terminal with LCI-KR2 so that the expression of LCI-KR2 will be reported.
Characterization
1. Identification
After receiving the synthesized DNA, PCR was done to certify that the plasmid was correct, and the experimental results were shown in figure 1.
- Fig. 1. Gene circuit involved in protein expression and AGE analysis of string a, Gene circuit was constructed by BBa_K3739020 to express LCIKR-2-GFP. b, Target bands of LCIKR-2-GFP (black arrow, 1200 bp).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 199