Difference between revisions of "Part:BBa K3777029"
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Revision as of 18:01, 21 October 2021
luxR-Plux-dCas9-mphA
A biosensor device that can improve the detection limit of erythromycin.
Usage and Biology
Compared to our another part BBa_K3777028,this part add a gene called mphA, which can improve the detection limit of erythromycin
Referernce:Miller Corwin A,Ho Joanne M,Parks Sydney E,Bennett Matthew R. Macrolide Biosensor Optimization through Cellular Substrate Sequestration.[J]. ACS synthetic biology,2021,10(2)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2204
Illegal NheI site found at 5364
Illegal NheI site found at 5387 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4483
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 5951
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1022