Difference between revisions of "Part:BBa K3790152"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 17:50, 21 October 2021
Bst-(G2S)3-E.ssb
Introduction
The fusion protein obtained by linking the C-terminus of Bst to the N-terminal of E.ssb via a (G2S)3 linker.
Usage and Biology
Experimental Results
The length of xxxx DNA was xxx bp, which is approximately xxx bp after adding homology arms to both ends for PCR cloning. We isolated the DNA of interest by gel extraction for subsequent reactions.
Figure 2. Assembled DNA binding proteins, albA1, S.ssb, E.ssb. The first lane was loaded with DNA ladder, sizes were marked on the image. The brightest band of 750 bp was about 100 ng, and other bands about 50 ng. Lanes with correct sized amplified DNA were labeled. After PCR cloning, several bacterial clones were picked, grew into cultures and sent for Sanger sequencing. Then, we verified the sequencing results, and used the correct ones for further experiments.
Reference
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]