Difference between revisions of "Part:BBa K4060516"

Line 3: Line 3:
 
<partinfo>BBa_K4060516 short</partinfo>
 
<partinfo>BBa_K4060516 short</partinfo>
  
The part that establishes the expression of BphP1, QPAS1, and HO1, and also contains a GFP reporter gene (BBa_K763004) that report the LexA signal in the cell. This part is for functional test of BphP1-LexA_QPAS1 optogenetic system
+
The part that establishes the expression of BphP1, QPAS1, and HO1, and also contains a GFP reporter gene (BBa_K763004) that report the LexA signal in the cell. This part is for functional test of BphP1-QPAS1 optogenetic system
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:20, 21 October 2021


BBa_K4060513 + BBa_K4060514 + BBa_K4060504

The part that establishes the expression of BphP1, QPAS1, and HO1, and also contains a GFP reporter gene (BBa_K763004) that report the LexA signal in the cell. This part is for functional test of BphP1-QPAS1 optogenetic system

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 144
    Illegal NheI site found at 167
    Illegal NheI site found at 2551
    Illegal NheI site found at 2574
    Illegal NheI site found at 3215
    Illegal NheI site found at 3238
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 619
    Illegal XhoI site found at 2369
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 963
    Illegal PstI site found at 1260
    Illegal NgoMIV site found at 282
    Illegal NgoMIV site found at 570
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 1853
    Illegal NgoMIV site found at 2355
    Illegal NgoMIV site found at 2761
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1410
    Illegal BsaI.rc site found at 2256