Difference between revisions of "Part:BBa K3790150"

Revision as of 16:58, 21 October 2021

Bst-(G2S)3-DbpA

Introduction

The fusion protein obtained by linking the C-terminus of Bst Pol to the N-terminal of DbpA via a (G2S)3 linker

Usage and Biology

Experimental Results

The length of xxxx DNA was xxx bp, which is approximately xxx bp after adding homology arms to both ends for PCR cloning. We isolated the DNA of interest by gel extraction for subsequent reactions.

Figure 2. Assembled DNA binding proteins, albA1, S.ssb, E.ssb. The first lane was loaded with DNA ladder, sizes were marked on the image. The brightest band of 750 bp was about 100 ng, and other bands about 50 ng. Lanes with correct sized amplified DNA were labeled. After PCR cloning, several bacterial clones were picked, grew into cultures and sent for Sanger sequencing. Then, we verified the sequencing results, and used the correct ones for further experiments.

Reference

Sequence and Features