Difference between revisions of "Part:BBa K239001:Design"

 
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===References===
 
===References===
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The BaeSR two-component regulatory system mediates resistance to condensed tannins in Escherichia coli.
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Zoetendal EG, Smith AH, Sundset MA, Mackie RI. 2008
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Appl Environ Microbiol. 2008 Jan;74(2):535-9. Epub 2007 Nov 26.

Revision as of 10:47, 29 June 2009

Spy promoter, activated by Phosporylated CpxR, BaeR and Sigma70


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first BaeR binding site and ends 5 pb after the -10 box.


Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

References

The BaeSR two-component regulatory system mediates resistance to condensed tannins in Escherichia coli. Zoetendal EG, Smith AH, Sundset MA, Mackie RI. 2008 Appl Environ Microbiol. 2008 Jan;74(2):535-9. Epub 2007 Nov 26.