Difference between revisions of "Part:BBa K239005:Design"

 
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===References===
 
===References===
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Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum. Kolesnikow T, Schröder I, Gunsalus RP.
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J Bacteriol. 1992 Nov;174(22):7104-11.

Revision as of 10:11, 29 June 2009

NarK promoter, Fnr activated under anaerobic conditions


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the IHF binding site and ends 6 pb after the -10 box.


Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

References

Regulation of narK gene expression in Escherichia coli in response to anaerobiosis, nitrate, iron, and molybdenum. Kolesnikow T, Schröder I, Gunsalus RP. J Bacteriol. 1992 Nov;174(22):7104-11.