Difference between revisions of "Part:BBa K3764002"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3764002 short</partinfo>
 
<partinfo>BBa_K3764002 short</partinfo>
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This part is s modified version of a part E1010. The codon for Tyr at position 38 is changed to Amber stop codon (TAG).
 
This part is s modified version of a part E1010. The codon for Tyr at position 38 is changed to Amber stop codon (TAG).

Revision as of 16:25, 21 October 2021


mRFP1 with T38stop (TAG) mutation


This part is s modified version of a part E1010. The codon for Tyr at position 38 is changed to Amber stop codon (TAG).


It can be used as part of a sensor for Amber codon suppression experiments. Moreover, our team (iGEM Bulgaria 2021) used this part in combination with BBa_K3764002 to design a cloning-based system for monitoring the formation of unproductive chimeras and artifacts between highly similar DNA sequences in PCR amplifications.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]