Difference between revisions of "Part:BBa K4046800"

Revision as of 16:24, 21 October 2021

CMV - BS #1 - Kozak - cLuc - bghA

This is a binding site for the DhdR gene, as identified in the literature by Xiao et al. In this composite part, we added a Kozak sequence and cLuc gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG. For more information about the expected behavior of the circuit, please consult the “Design Considerations section.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 614
Illegal BglII site found at 1257
Illegal BglII site found at 1316
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4046800 short</partinfo>
-This is a binding site for the DhdR gene, as identified in the literature. In this composite part, we added a Kozak sequence and cLuc gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG.
+This is a binding site for the DhdR gene, as identified in the literature by Xiao et al. In this composite part, we added a Kozak sequence and <i>cLuc</i> gene downstream of the binding site and promoter, which should allow for the part to be used in a DhdR-based biosensor for oncometabolite D-2-HG. For more information about the expected behavior of the circuit, please consult the “Design Considerations'' section.
 <!-- Add more about the biology of this part here