Difference between revisions of "Part:BBa K3905002"
 
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Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa210-3p is present, the miRNA binds to part BBa_3905003 (gen1 210-3p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.
 
Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa210-3p is present, the miRNA binds to part BBa_3905003 (gen1 210-3p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.
  
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<img width="800px" src="https://2021.igem.org/wiki/images/thumb/6/64/T--City_of_London_UK--Toehold_Switch3.png/1200px-T--City_of_London_UK--Toehold_Switch3.png">
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Locked and labelled toehold switch.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:23, 21 October 2021


gen1 210-3p Toehold Switch

Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa210-3p is present, the miRNA binds to part BBa_3905003 (gen1 210-3p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]