Difference between revisions of "Part:BBa K3905004"

 
Line 4: Line 4:
  
 
This Toehold Switch is activated by hsa-MiRNA 517-5p. The micro RNA 517-5p alone acts as the trigger for the toehold switch and causes the riboregulator to unfold, exposing the RBS and start codon. Placing a reporter protein downstream of this part will mean that when miRNA 517-5p is present in high enough quantities, the protein will be translated and the reporter protein can be observed.   
 
This Toehold Switch is activated by hsa-MiRNA 517-5p. The micro RNA 517-5p alone acts as the trigger for the toehold switch and causes the riboregulator to unfold, exposing the RBS and start codon. Placing a reporter protein downstream of this part will mean that when miRNA 517-5p is present in high enough quantities, the protein will be translated and the reporter protein can be observed.   
 +
 +
<html>
 +
<figure>
 +
<img width="800px" src="https://2021.igem.org/wiki/images/thumb/d/d6/T--City_of_London_UK--Toehold_Switch1.png/1200px-T--City_of_London_UK--Toehold_Switch1.png">
 +
</figure>
 +
<figcaption>
 +
<i>
 +
Labelled toehold switch in its 'off' state.
 +
<i>
 +
</figcaption>
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:18, 21 October 2021


gen2 517-5p Toehold Switch

This Toehold Switch is activated by hsa-MiRNA 517-5p. The micro RNA 517-5p alone acts as the trigger for the toehold switch and causes the riboregulator to unfold, exposing the RBS and start codon. Placing a reporter protein downstream of this part will mean that when miRNA 517-5p is present in high enough quantities, the protein will be translated and the reporter protein can be observed.

Labelled toehold switch in its 'off' state.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]