Difference between revisions of "Part:BBa K3792002"
 
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__NOTOC__
 
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<partinfo>BBa_K3792002 short</partinfo>
 
<partinfo>BBa_K3792002 short</partinfo>
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<img src="https://2021.igem.org/File:T--FSU--NodCIJOperon.png">
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NodC, NodI and NodJ in sequence. Each preceded by an RBS.   
 
NodC, NodI and NodJ in sequence. Each preceded by an RBS.   
  

Latest revision as of 16:13, 21 October 2021


NodCIJ Operon

NodC, NodI and NodJ in sequence. Each preceded by an RBS.

NodC is the Chitin synthase from Rhiozobium leguminosarum, Nodl a protein possibly involved in transporting chitin across the cell membrane, and NodJ, a protein also possibly involved in the transportation of chitin

To test if the cells were secreting chitin, The FSU 2021 team used the Chitosan Assay Kit (XAN-5126) available from Cell Biolabs. The manual for this kit contains the protocols for converting chitin to chitosan, along with a protocol for detecting chitosan.

Chitin from the cells was converted into chitosan by reacting with 12.5M NaOH at 95°C for 24 hours. The solution was then neutralized with acetic acid. The assay was then performed on the supernatant of the cell culture.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 385
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 747