Difference between revisions of "Part:BBa K3905000"
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Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.
 
Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.
  
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<img width="800px" src="https://2021.igem.org/wiki/images/thumb/0/02/T--City_of_London_UK--Complex_P2.png/180px-T--City_of_London_UK--Complex_P2.png">
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Diagram of 1st generation toehold switch in action.
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Revision as of 16:01, 21 October 2021


gen1 517-5p Toehold Switch

Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.

Diagram of 1st generation toehold switch in action.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 15
    Illegal XbaI site found at 53
  • 1000
    COMPATIBLE WITH RFC[1000]