Difference between revisions of "Part:BBa K3805209"

Revision as of 15:41, 21 October 2021

Bi-stable Switch

This part is a toggle switch.It is composed of trc promoter(BBa_K3805208))[1], P2 promoter(BBa_I746104))[2], lacI- LVA (BBa_C0012))[3]and ompT-mcherry(BBa_K3805886))[4].

Usage and Biology

At the presence of autoinducing peptides(AIPs), P2 promoter will be activated by phosphorylated AgrA (BBa_K38055))[5]and initiate the expression of downstrem lacI-LVA and ompT. LacI-LVA inhibits trc promoter.As the concentration of AIP decreases, the downstream transcription of P2 promoter will be down-regulated and the inhibition of lacI-LVA on the trc promoter will thus be lifted.The trc promoter then initiates the its downstream expression.

Figure 1 Images under fluorescence microscope.(A)Before putting into AIPs, red fluorescence was observed.(B).After putting into AIP, red fluorescence disappeared,green fluorescence was observed. (fluorescence microscope 10x100)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1287
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:38, 21 October 2021 (view source) Wangwang (Talk \| contribs) ← Older edit		Revision as of 15:41, 21 October 2021 (view source) Wangwang (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K3805209 short</partinfo>		<partinfo>BBa_K3805209 short</partinfo>

−	This part is ~~an bistable~~ switch.It is composed of trc promoter(BBa_K3805208))[https://parts.igem.org/Part:BBa_K3805208], P2 promoter(BBa_I746104))[https://parts.igem.org/Part:BBa_I746104], lacI- LVA (BBa_C0012))[https://parts.igem.org/Part:BBa_C0012]and ompT-mcherry(BBa_K3805886))[https://parts.igem.org/Part:BBa_K3805886].	+	This part is a toggle switch.It is composed of trc promoter(BBa_K3805208))[https://parts.igem.org/Part:BBa_K3805208], P2 promoter(BBa_I746104))[https://parts.igem.org/Part:BBa_I746104], lacI- LVA (BBa_C0012))[https://parts.igem.org/Part:BBa_C0012]and ompT-mcherry(BBa_K3805886))[https://parts.igem.org/Part:BBa_K3805886].