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| − | ===Applications of BBa_K3788025===
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| − | ===Cell visualization with confocal microscope===
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| − | <p>We wanted to determine whether the fluorescence is located in the periplasm or in the cytoplasm, so we used a confocal microscope to visualize our cells.</p>
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| − | <p>We transformed E.Coli MG1655 strains with an empty J04500 vector or with the BBa_K3788025 recombinant plasmid. We then prepared 3mL 2YT growing medium with chloramphenicol and then put MG1655 containing either J04500 or BBa_K3788025 plasmids.</p>
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| − | <p>After 3 hours of incubation at 37°C, we prepared the plates and visualized our cells </p>
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| − | https://2021.igem.org/wiki/images/d/d6/T--Aix-Marseille--Microscopie_J04500.jpeg
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| − | <i><b>Figure :</b> Visualisation of MG1655 E.coli strains containing J04500</i>
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| − | https://2021.igem.org/wiki/images/3/31/T--Aix-Marseille--Microscopie_J04500%2BPelBsfGFP.jpeg
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| − | <i><b>Figure :</b> Visualisation of MG1655 E.coli strains containing BBa_K3788025</i>
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| − | <p>We can see that bacteria containing J04500 do not emit light, contrary to the ones containing J04500_PelBsfGFP, which are green under a confocal microscope.</p>
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| − | <p>However, we are not able to determine whether the light is located at the surface of the bacteria or if it is located in all the cells.</p>
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| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 15:35, 21 October 2021
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