Difference between revisions of "Part:BBa K3735888"

 
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<partinfo>BBa_K3735888 short</partinfo>
 
<partinfo>BBa_K3735888 short</partinfo>
  
BBa_K3735001 and BBa_K3735002 are the composite parts which can improve the SeNPs stability and increase the reduction rate of selenite respectively. We combined these two composite parts to form BBa_K3735017 which is vital to efficient synthesis of high-stability SeNPs.
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In order to explore the optimal conditions for the production of SeNPs for BBa_K3735004, we used the orthogonal experimental model-Response surface model(more details see CO Model).
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We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments.
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<img src="https://2021.igem.org/wiki/images/4/47/T--NAU-CHINA--Project_Results.fig.%E5%93%8D%E5%BA%94%E9%9D%A2%E9%A2%84%E5%AE%9E%E9%AA%8C.png"width="700" height=""width="350" height=""/>
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<p style="text-align: center!important;"><b>Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16°C,30°C,37°C and selenite concentration are 12mM,15mM,18mM. </b></p>
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Second according to preliminary results and setting gradients to explore the interaction between three different types of conditions—pH 6, 7, and 8, temperature 16。C, 30。C, and 37。C, selenite concentration 12, 15, and 18mM, and conducted experiments as shown in the Fig.2.</p>
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<img src="https://2021.igem.org/wiki/images/0/04/T--NAU-CHINA--Project_Results.fig.6.png"width="700" height=""width="350" height=""/>
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<p style="text-align: center!important;"><b>Fig.2 Response surface experiment of SeNPs synthesis. </b></p>
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<p>We used response surface model to simulate the optimal SeNPs production conditions (details see <u>CO Model</u>(此处添加超链接至相关页面)). Finally, we obtained the optimal conditions and maximum yield as follows:
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<p>Solutions</p>
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<img src="https://2021.igem.org/wiki/images/d/d5/T--NAU-CHINA--Project_Results.fig.7%28%E8%BF%99%E6%AC%A1%E5%8F%AF%E4%BB%A5%E4%BA%86%E5%90%A7%29.png"width="700" height=""width="350" height=""/>
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<p style="text-align: center!important;"><b>Fig.3 the final product of our project. </b></p>
  
  

Revision as of 15:34, 21 October 2021


SeNPs production parts

In order to explore the optimal conditions for the production of SeNPs for BBa_K3735004, we used the orthogonal experimental model-Response surface model(more details see CO Model). We hope to maximize SeNPs production by optimizing temperature, pH and selenite concentration. First, we conducted a preliminary experiment on these three factors, and we chose the best three gradients(Fig.1) of these three factors to do further experiments.

Fig.1 Preliminary experiment results of response surface experiment. A: pH; B: concentration of selenite; C: Temperature. The results of preliminary experiment show that the SeNPs yield is the best when pH are 6,7,8 temperature are 16°C,30°C,37°C and selenite concentration are 12mM,15mM,18mM.

Second according to preliminary results and setting gradients to explore the interaction between three different types of conditions—pH 6, 7, and 8, temperature 16。C, 30。C, and 37。C, selenite concentration 12, 15, and 18mM, and conducted experiments as shown in the Fig.2.</p>

Fig.2 Response surface experiment of SeNPs synthesis.

We used response surface model to simulate the optimal SeNPs production conditions (details see CO Model(此处添加超链接至相关页面)). Finally, we obtained the optimal conditions and maximum yield as follows: <p>Solutions

Fig.3 the final product of our project.


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