Difference between revisions of "Part:BBa K3805329"
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<partinfo>BBa_K3805329 short</partinfo> | <partinfo>BBa_K3805329 short</partinfo> | ||
| − | We combined three kinds of Human insulin proteinogen(https://parts.igem.org/Part:BBa_K3805429, https://parts.igem.org/Part:BBa_K3805001, https://parts.igem.org/Part:BBa_K3805001) with AraC(https://parts.igem.org/Part:BBa_I0500), let Human insulin proteinogen sequence affect the expression of AraC. | + | We combined three kinds of Human insulin proteinogen(https://parts.igem.org/Part:BBa_K3805429, https://parts.igem.org/Part:BBa_K3805001, https://parts.igem.org/Part:BBa_K3805001) with AraC(https://parts.igem.org/Part:BBa_I0500), let Human insulin proteinogen sequence affect the expression of AraC. Here we take human insulin proteinogen(Normal) as an example. |
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Revision as of 15:26, 21 October 2021
Fusion protein with proinsulin and araC
We combined three kinds of Human insulin proteinogen(https://parts.igem.org/Part:BBa_K3805429, https://parts.igem.org/Part:BBa_K3805001, https://parts.igem.org/Part:BBa_K3805001) with AraC(https://parts.igem.org/Part:BBa_I0500), let Human insulin proteinogen sequence affect the expression of AraC. Here we take human insulin proteinogen(Normal) as an example.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1487
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1426
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1261
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1243