Difference between revisions of "Part:BBa K3814023"

Latest revision as of 14:55, 21 October 2021

5' Homology Arm for Cluster 5

This 50bp section of DNA is random DNA that is going to be used as a homology arm for recombineering. As part of USYD 2021's project, we have developed a novel recombineering strategy (Babushka Blocks) to insert large amounts of linear DNA into the E. coli chromosome. This and all other homology arms listed in the parts registry can be used by future teams. This homology arm have been designed to minimise self-dimerisation and hairpin formations, as in USYD 2021's project, it will also exist as a separate primer.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:03, 1 October 2021 (view source) Registry (Talk \| contribs)		Latest revision as of 14:55, 21 October 2021 (view source) Simontang (Talk \| contribs)
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	<partinfo>BBa_K3814023 short</partinfo>		<partinfo>BBa_K3814023 short</partinfo>

−	This 50bp section of DNA is going to be used as a homology arm to insert ~~the next gene cluster~~ into. ~~These bases~~ have been designed to minimise self-dimerisation and hairpin formations, as it will also exist as a separate primer.	+	This 50bp section of DNA is random DNA that is going to be used as a homology arm for recombineering. As part of USYD 2021's project, we have developed a novel recombineering strategy (Babushka Blocks) to insert large amounts of linear DNA into the E. coli chromosome. This and all other homology arms listed in the parts registry can be used by future teams. This homology arm have been designed to minimise self-dimerisation and hairpin formations, as in USYD 2021's project, it will also exist as a separate primer.