Difference between revisions of "Part:BBa K3788017:Experience"
| Line 4: | Line 4: | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | <h1>Applications of BBa_K3788017</h1> |
| + | ===Methods=== | ||
| + | <p>In order to characterize the promoter strength, we fused it to the GFP encoding sequence BBa_E0040 and to the timer-lysis device part <b>BBa_K3788018</b> (composite part <b>BBa_K3788019</b>). Induction of the SOS response was induced by mitomycin C treatments and the fluorescence was recorded in a dose-dependent manner using a microplate reader. Following iGEM Measurement recommendations, the GFP fluorescence intensity was converted to a concentration of fluorescein protein thanks to a standard curve of fluorescein dilution series.</p> | ||
| + | <br> | ||
| + | ===Results=== | ||
| + | <p> The cells carrying the <b>BBa_K3788019</b> construct were grown with various concentrations of mitomycin, ranging from 0 to 800 ng.ml-1. The OD (600 nm) and the amount of green fluorescence were recorded over time.</p> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 14:32, 21 October 2021
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K3788017
Methods
In order to characterize the promoter strength, we fused it to the GFP encoding sequence BBa_E0040 and to the timer-lysis device part BBa_K3788018 (composite part BBa_K3788019). Induction of the SOS response was induced by mitomycin C treatments and the fluorescence was recorded in a dose-dependent manner using a microplate reader. Following iGEM Measurement recommendations, the GFP fluorescence intensity was converted to a concentration of fluorescein protein thanks to a standard curve of fluorescein dilution series.
Results
The cells carrying the BBa_K3788019 construct were grown with various concentrations of mitomycin, ranging from 0 to 800 ng.ml-1. The OD (600 nm) and the amount of green fluorescence were recorded over time.
User Reviews
UNIQ708950986b86e2b5-partinfo-00000000-QINU UNIQ708950986b86e2b5-partinfo-00000001-QINU