Difference between revisions of "Part:BBa K3764000"

Revision as of 14:21, 21 October 2021

Module for generation of a T-vector

This part contains a specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion. Moreover, this part also supports the blue-white screening procedure available for vectors like pUC19 and pBluescript. Once integrated into a BioBrick vector it will transform the corresponding plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE SELECTED VECTOR BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.

This part has no promoter! You should add one if you want to use the blue-white screening procedure with your newly generated T-vector.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 341
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 335
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3764000 short</partinfo>
-This part contains a specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion. Once integrated into a BioBrick vector it will transform the plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE SELECTED VECTOR BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.
+This part contains a specialized module for the generation of a T-vector upon Eam1105I (AhdI) digestion. Moreover, this part also supports the blue-white screening procedure available for vectors like pUC19 and pBluescript. Once integrated into a BioBrick vector it will transform the corresponding plasmid into a TA cloning compatible one. PLEASE BEFORE USE CHECK IF THE SELECTED VECTOR BACKBONE CONTAINS A Eam1105I (AhdI) RECOGNITION SITE. If this is the case, all such sites should be mutagenized before obtaining a functional T-vector.
 This part has no promoter! You should add one if you want to use the blue-white screening procedure with your newly generated T-vector.