Difference between revisions of "Part:BBa K3776011"
 
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<partinfo>BBa_K3776011 short</partinfo>
 
<partinfo>BBa_K3776011 short</partinfo>
  
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This crRNA complementary to the neutral site 2 (NS2) in the chromosome Synechococcus elongatus UTEX 2973.  
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This is Neutral Site I (NSII) of S.elongatus UTEX 2973. Neutral sites are sites in the chromosome commonly used as sites for gene manipulation in S.elongatus as disrupting these sites does not cause discernible phenotype(Rosato, 2010).
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Our team is creating a crRNA targeting NSII for CRISPR technology to edit the genome of S.elongatus 2973.  
  
 
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Latest revision as of 14:19, 21 October 2021


NSII

This is Neutral Site I (NSII) of S.elongatus UTEX 2973. Neutral sites are sites in the chromosome commonly used as sites for gene manipulation in S.elongatus as disrupting these sites does not cause discernible phenotype(Rosato, 2010). Our team is creating a crRNA targeting NSII for CRISPR technology to edit the genome of S.elongatus 2973.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1119
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1119
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1119
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1119
  • 1000
    COMPATIBLE WITH RFC[1000]