Difference between revisions of "Part:BBa K3776010"
 
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<partinfo>BBa_K3776010 short</partinfo>
 
<partinfo>BBa_K3776010 short</partinfo>
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This is Neutral Site I (NSI) of S.elongatus UTEX 2973. Neutral sites are sites in the chromosome commonly used as sites for gene manipulation in S.elongatus as disrupting these sites does not cause discernible phenotype(Rosato, 2010).
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This crRNA complementary to the neutral site 1 (NS1) in the chromosome Synechococcus elongatus UTEX 2973.  
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Our team is creating a crRNA targeting NSI for CRISPR technology to edit the genome of S.elongatus 2973.  
  
 
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Latest revision as of 14:18, 21 October 2021


NSI This is Neutral Site I (NSI) of S.elongatus UTEX 2973. Neutral sites are sites in the chromosome commonly used as sites for gene manipulation in S.elongatus as disrupting these sites does not cause discernible phenotype(Rosato, 2010). Our team is creating a crRNA targeting NSI for CRISPR technology to edit the genome of S.elongatus 2973.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 604
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 791
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 604
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 604
    Illegal NgoMIV site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]