Difference between revisions of "Part:BBa K4012021"
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[[Image:yeast PCR.jpg|thumbnail|750px|center|'''Figure 3:''' | [[Image:yeast PCR.jpg|thumbnail|750px|center|'''Figure 3:''' | ||
[https://parts.igem.org/Part:BBa_K4012021] A: The results of colony PCR of upstream transformant; B: The results of colony PCR of downstream transformant; C: Construction of catechin metabolic pathway; D:Construction of naringenin metabolic pathway]] | [https://parts.igem.org/Part:BBa_K4012021] A: The results of colony PCR of upstream transformant; B: The results of colony PCR of downstream transformant; C: Construction of catechin metabolic pathway; D:Construction of naringenin metabolic pathway]] | ||
− | tENO2 is also involved in assembly of synthesis pathway of | + | tENO2 is also involved in assembly of synthesis pathway of catechin, shown by Fig.3. |
Latest revision as of 14:14, 21 October 2021
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tENO2
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Secondary Structure
Measurement
- [http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]
Obtaining the fragment and BsaI digested verification
![](/wiki/images/0/09/T--AISSU_Union--partslast.jpg)
Figure 1: [1] Results of yeast toolkit plasmids enzyme-digested verification
We construct tENO2 with vector type8-pSB1K3-GFP and proceed digested verification using BsaI. According to Fig.1, we obtain two clear bands after BsaI digested, the length of the vector(1622bp) and the inserted fragments tENO2(238bp) matched with previous expectations by compared with Marker MK8000 ladder, confirming the successful assembly of Toolkit plasmids and availability in subsequent construction.
tENO2 in Level1 plasmid assembly
![](/wiki/images/6/63/CDS_CsF3H.jpg)
Figure 2: [https://parts.igem.org/Part:BBa_K4012021
tENO2 in Level2 plasmid assembly
![](/wiki/images/0/0d/Yeast_PCR.jpg)
Figure 3: [2] A: The results of colony PCR of upstream transformant; B: The results of colony PCR of downstream transformant; C: Construction of catechin metabolic pathway; D:Construction of naringenin metabolic pathway
tENO2 is also involved in assembly of synthesis pathway of catechin, shown by Fig.3.