Difference between revisions of "Part:BBa K3814004"

 
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__NOTOC__
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<partinfo>BBa_K3814004 short</partinfo>
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fuGFP, short for ‘free-use GFP’, is a patent-free GFP developed by Research Associate Mark Somerville and Associate Professor Nick Coleman in the Coleman Lab (Coleman & Somerville, 2019).
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Current GFPs like superfolding GFP (sfGFP) are protected IP and patented. This can make any commercial or even academic use of them expensive, and a barrier to research. Reading through the patents of GFPs, it was realised that if a GFP could be made with less than 80% homology to currently patented GFPs, it would escape patent protection!
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So off they worked, and eventually they came to a product that had “76% amino acid identity to GFPmut3”, well below the 80% goal! See below its fluorescence:
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[[File:T--Sydney_Australia--fugfp.png|500x500px|Caption]]
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'''Figure 1.''' Expression of fuGFP in E.coli strain TOP10.
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'''References'''
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Coleman, N., & Somerville, M. (2019, May). The Story of Free Use GFP (fuGFP). Small Things Considered. https://schaechter.asmblog.org/schaechter/2019/05/the-story-of-free-use-gfp-fugfp.html
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3814004 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K3814004 parameters</partinfo>
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Revision as of 14:10, 21 October 2021


free-use GFP (fuGFP)

fuGFP, short for ‘free-use GFP’, is a patent-free GFP developed by Research Associate Mark Somerville and Associate Professor Nick Coleman in the Coleman Lab (Coleman & Somerville, 2019).

Current GFPs like superfolding GFP (sfGFP) are protected IP and patented. This can make any commercial or even academic use of them expensive, and a barrier to research. Reading through the patents of GFPs, it was realised that if a GFP could be made with less than 80% homology to currently patented GFPs, it would escape patent protection!

So off they worked, and eventually they came to a product that had “76% amino acid identity to GFPmut3”, well below the 80% goal! See below its fluorescence:

Caption

Figure 1. Expression of fuGFP in E.coli strain TOP10.


References

Coleman, N., & Somerville, M. (2019, May). The Story of Free Use GFP (fuGFP). Small Things Considered. https://schaechter.asmblog.org/schaechter/2019/05/the-story-of-free-use-gfp-fugfp.html


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 151
  • 1000
    COMPATIBLE WITH RFC[1000]