Difference between revisions of "Part:BBa K3814005"
Line 3: | Line 3: | ||
<partinfo>BBa_K3814005 short</partinfo> | <partinfo>BBa_K3814005 short</partinfo> | ||
− | Psal promoter with adjustments to have tighter repression without salicylate and higher expression when fully induced. | + | Psal promoter with adjustments to have tighter repression without salicylate and higher expression when fully induced. |
− | Promoter | + | {| |
− | Psal | + | |'''Promoter''' |
− | PsalTTC | + | |'''-35''' |
+ | |'''-10''' | ||
+ | |- | ||
+ | |Psal | ||
+ | |TTATCA | ||
+ | |TATCGT | ||
+ | |- | ||
+ | |PsalTTC | ||
+ | |TTATTT | ||
+ | |TACCGT | ||
+ | |} | ||
+ | |||
+ | |||
+ | This example showcases a variant that yields 34% expression levels of WT Psal promoters. This is described more indepth in the design section. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 14:02, 21 October 2021
Psal1 (PsalTTC, 34% Activity)
Psal promoter with adjustments to have tighter repression without salicylate and higher expression when fully induced.
Promoter | -35 | -10 |
Psal | TTATCA | TATCGT |
PsalTTC | TTATTT | TACCGT |
This example showcases a variant that yields 34% expression levels of WT Psal promoters. This is described more indepth in the design section.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 68
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]