Difference between revisions of "Part:BBa K3814002"

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|Promoter
 
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|Psal
 
|Psal

Revision as of 14:01, 21 October 2021


Psal1 (PsalTTC, 100% Activity)

Psal promoter with adjustments to have tighter repression without salicylate and higher expression when fully induced.

Promoter "-35" "-10"
Psal TTATCA TATCGT
PsalTTC TTATTT TACCGT


This example showcases a variant that yields 34% expression levels of WT Psal promoters. This is described more indepth in the design section.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 68
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]