Difference between revisions of "Part:BBa K4055533"
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<partinfo>BBa_K4055533 short</partinfo>
 
<partinfo>BBa_K4055533 short</partinfo>
  
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Through the combination of three components, we made a blue light photosensitive system. When exposed to blue light,this part can activate the following T7 RNA polymerase.
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fused protein
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<p>
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In particular,in this circuit, the expression of histidinine kinase YF1 and its homologous regulatory factor FixJ are tightly regulated by LacIq promoter, while the expression repressor cI of phage λ is controlled by pFixK2 promoter. Under blue light irradiation, the kinase activity and subsequent cI expression of YF1 were inhibited, which then activated the λ promoter pR and promoted the expression of CSGA-MFP3S-PEP.
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</p>
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===Experiment===
 
===Experiment===
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Experiments in BBa_K4055535 are used to prove the effectiveness of the system
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Experiments in [[Part:BBa_K4055531]] are used to prove the effectiveness of the system
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:52, 21 October 2021


CsgA-Mfp3S-pep

fused protein

Experiment

Experiments in Part:BBa_K4055531 are used to prove the effectiveness of the system

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]