Difference between revisions of "Part:BBa K3905000"

Revision as of 13:33, 21 October 2021

gen1 517-5p Toehold Switch

Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 15
Illegal XbaI site found at 53
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 15
Illegal XbaI site found at 53
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 15
Illegal XbaI site found at 53
1000
COMPATIBLE WITH RFC[1000]

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 Toehold switch that regulates the translation of a protein, positively induced by a trigger RNA. When micro-RNA hsa517-5p is present, the miRNA binds to part BBa_3905001 (gen1 517-5p Anti-miRNA) which forms a duplex. This duplex trigger causes the toehold switch to unfold by binding to the trigger binding site, exposing the RBS and start codon and allowing translation to occur at a ribosome. In a composite, a reporter gene should be placed downstream here which will report when in the 'on' state.
-[[File:T--City of London UK--Complex P1.png|none|200px|thumb|left|diagram of switch in action.]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===