Difference between revisions of "Part:BBa K3921007"
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In order to verify that sRNA DicF can indeed inhibit the cell division of MG1655, we construct this circuit. | In order to verify that sRNA DicF can indeed inhibit the cell division of MG1655, we construct this circuit. | ||
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When induced by arabinose, DicF was expressed and MG1655 cell division was inhibited. See BBa_K3921006 for detailed experimental results. | When induced by arabinose, DicF was expressed and MG1655 cell division was inhibited. See BBa_K3921006 for detailed experimental results. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 13:17, 21 October 2021
araC/pBAD-dicF-T1/T2
In order to verify that sRNA DicF can indeed inhibit the cell division of MG1655, we construct this circuit.
When induced by arabinose, DicF was expressed and MG1655 cell division was inhibited. See BBa_K3921006 for detailed experimental results.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961