Difference between revisions of "Part:BBa K3805110"

 
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<partinfo>BBa_K3805110 short</partinfo>
 
<partinfo>BBa_K3805110 short</partinfo>
  
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By fusing proinsulin with AraC, when the fusion protein is normally expressed, that is, when insulin is normally expressed, the fusion protein inhibits the opening of PBAD promoter and the expression of its downstream pathway. On the contrary, when the fusion protein is not expressed, this part can reflect the presence or absence of the target product.
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BBa_K3805110 consists of two parts: mRNA binding site(https://parts.igem.org/Part:BBa_K3805639) and yeGFP(https://parts.igem.org/Part:BBa_K3402000), the mRNA binding site can affect the expression of GFP by binding to complementary mRNAs.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:46, 21 October 2021


mRNA switch

BBa_K3805110 consists of two parts: mRNA binding site(https://parts.igem.org/Part:BBa_K3805639) and yeGFP(https://parts.igem.org/Part:BBa_K3402000), the mRNA binding site can affect the expression of GFP by binding to complementary mRNAs.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 20
    Illegal SpeI site found at 40
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 40
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 58
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 20
    Illegal SpeI site found at 40
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 20
    Illegal SpeI site found at 40
  • 1000
    COMPATIBLE WITH RFC[1000]