Difference between revisions of "Part:BBa K4011007"

 
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SaACS2 is an acetyl-CoA synthase, responsible for synthesizing acetyl-CoA from a host of different substrates. This year, we will express SaACS2 in yeast cells to turn acetate into acetyl-CoA, providing the first step for our metabolic pathway which converts acetate and ethanol into ethyl-acetate. The part will be used to construct yeast expression plasmids through golden gate assembly into pTEF-SaACS2-tADH1<partinfo>BBaK4011016</partinfo> and pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1 <partinfo>BBaK4011019</partinfo>.
 
SaACS2 is an acetyl-CoA synthase, responsible for synthesizing acetyl-CoA from a host of different substrates. This year, we will express SaACS2 in yeast cells to turn acetate into acetyl-CoA, providing the first step for our metabolic pathway which converts acetate and ethanol into ethyl-acetate. The part will be used to construct yeast expression plasmids through golden gate assembly into pTEF-SaACS2-tADH1<partinfo>BBaK4011016</partinfo> and pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1 <partinfo>BBaK4011019</partinfo>.
  
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==Usage and Biology==
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SaACS2 is an acetyl-CoA synthase from <i>Salmonella enterica</i>. Its natural function is to convert acetate into acetyl-CoA under anaerobic conditions. Its enzymatic rate is around 50 times that of ACS1 found in <i>S. cerevisiae</i>.
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===Source===
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SaACS2 is from <i>Salmonella enterica</i>.
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==Characterization==
  
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===Usage and Biology===
 
  
 
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Revision as of 12:41, 21 October 2021


SaACS2

SaACS2 is an acetyl-CoA synthase, responsible for synthesizing acetyl-CoA from a host of different substrates. This year, we will express SaACS2 in yeast cells to turn acetate into acetyl-CoA, providing the first step for our metabolic pathway which converts acetate and ethanol into ethyl-acetate. The part will be used to construct yeast expression plasmids through golden gate assembly into pTEF-SaACS2-tADH1BBa_K4011016 and pTEF1-SaACS2-tADH1-pRPL8B-AeAT9-tSSA1 BBa_K4011019.

Usage and Biology

SaACS2 is an acetyl-CoA synthase from Salmonella enterica. Its natural function is to convert acetate into acetyl-CoA under anaerobic conditions. Its enzymatic rate is around 50 times that of ACS1 found in S. cerevisiae.

Source

SaACS2 is from Salmonella enterica.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1537
  • 1000
    COMPATIBLE WITH RFC[1000]