Difference between revisions of "Part:BBa K4012007"

 
 
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<partinfo>BBa_K4012007 parameters</partinfo>
 
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===Obtaining the pTEF2 fragment and BsaI digested verification===
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[[Image:T--AISSU_Union--partslast.jpg|thumbnail|750px|center|'''Figure 1:'''
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[https://parts.igem.org/Part:BBa_K4012007] Results of yeast toolkit plasmids enzyme-digested verification]]
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We construct pTEF2 with vector type8-pSB1K3-GFP and proceed digested verification using BsaI. According to Fig.1, we obtain two clear bands after BsaI digested, the length of the vector(1622bp) and the inserted fragments pTEF2(709bp) matched with previous expectations by compared with Marker MK8000 ladder, confirming the successful assembly of Toolkit plasmids and availability in subsequent construction.

Latest revision as of 11:31, 21 October 2021


pTEF2

it is a promotor that starts the sequence that is resposible for the production of chalcone synthase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 714
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 203
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 724


Obtaining the pTEF2 fragment and BsaI digested verification

Figure 1: [1] Results of yeast toolkit plasmids enzyme-digested verification

We construct pTEF2 with vector type8-pSB1K3-GFP and proceed digested verification using BsaI. According to Fig.1, we obtain two clear bands after BsaI digested, the length of the vector(1622bp) and the inserted fragments pTEF2(709bp) matched with previous expectations by compared with Marker MK8000 ladder, confirming the successful assembly of Toolkit plasmids and availability in subsequent construction.