Difference between revisions of "Part:BBa K3805924"
 
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<partinfo>BBa_K3805924 short</partinfo>
 
<partinfo>BBa_K3805924 short</partinfo>
  
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We combined AgrC(BBa_K3805508)[ https://parts.igem.org/Part:BBa_K3805525] and AgrA(BBa_K3805525)[https://parts.igem.org/Part:BBa_K3805508] together with T7 promoter and RBS, in order to form the two component system that can manipulate the activation of P2.promoter.
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We combined AgrC(BBa_K3805508)[https://parts.igem.org/Part:BBa_K3805525] and AgrA(BBa_K3805525[https://parts.igem.org/Part:BBa_K3805508] together with T7 promoter and RBS, in order to form the two component system that can manipulate the activation of P2.promoter.
  
  

Latest revision as of 11:02, 21 October 2021


T7-RBS1- AgrC-T7 terminator-RBS2- AgrA-T7Te terminator

We combined AgrC(BBa_K3805508)[1] and AgrA(BBa_K3805525[2] together with T7 promoter and RBS, in order to form the two component system that can manipulate the activation of P2.promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 814
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 814
    Illegal SpeI site found at 1355
  • 1000
    COMPATIBLE WITH RFC[1000]