Difference between revisions of "Part:BBa K3815000"
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<partinfo>BBa_K3815000 short</partinfo>
 
<partinfo>BBa_K3815000 short</partinfo>
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[[File:ELK16.png|200px|right|thumb|Fig1.ELK16]]
 
This part is for new purification method, ELK16 system.
 
This part is for new purification method, ELK16 system.
 
This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein. In this system, we don't have to need the expensive column or could save time removing tags.<br>
 
This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein. In this system, we don't have to need the expensive column or could save time removing tags.<br>

Revision as of 10:34, 21 October 2021


Mxe GryA intein-PT-linker-ELK16 for peptide production

Fig1.ELK16

This part is for new purification method, ELK16 system. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein. In this system, we don't have to need the expensive column or could save time removing tags.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 481
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173929/#MOESM2