Difference between revisions of "Part:BBa K3805886"

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<partinfo>BBa_K3805886 short</partinfo>
 
<partinfo>BBa_K3805886 short</partinfo>
  
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OmpT protease is highly expressed in E. coli, and the signal peptide of the amino-terminal 1 9 amino acid residues of its protein precursor is recognized, sheared and processed by the signal skin enzyme during transmembrane and then secreted into the peripheral lumen of the cell, so the signal skin sequence can be used to construct a secretory prokaryotic expression vector. By adding the ompT signal peptide sequence before the Mcherry gene expressed in the vector, the amino-terminal end of Mcherry can be secreted outside the cell under the guidance of the OmpT signal peptide.
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BBa_K3805886 consists of two parts: ompT signal peptide (BBa_K3805532)[ https://parts.igem.org/Part:BBa_K3805532] and mCherry (BBa_J06504) [https://parts.igem.org/Part:BBa_J06504]. By inserting the ompT signal peptide sequence at the upstream of mCherry gene, mCherry can be secreted to extracellular environment under the guidance of the OmpT signal peptide.
  
  

Revision as of 10:29, 21 October 2021


OmpT-mCherry

BBa_K3805886 consists of two parts: ompT signal peptide (BBa_K3805532)[ https://parts.igem.org/Part:BBa_K3805532] and mCherry (BBa_J06504) [1]. By inserting the ompT signal peptide sequence at the upstream of mCherry gene, mCherry can be secreted to extracellular environment under the guidance of the OmpT signal peptide.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]