Difference between revisions of "Part:BBa K3799057"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This is a high throughput sensing module that can sense the presence of AIP-I molecules. This part consists of pBAD promoter, AIP sensor | + | This is a high throughput sensing module that can sense the presence of AIP-I molecules. This part consists of a pBAD promoter, an AIP sensor infrastructure (BBa_I746101), an AIP inducible promoter P2 and a GFP reporter. |
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| + | In the natural system, the signalling <b>Auto-inducing peptide<b> (termed AIP) is made from AgrD, while AgrB, a transmembrane protein that is responsible for the cyclization. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 10:27, 21 October 2021
Bidirectional AIP-1 sensor
This is an improvement of the existing part BBa_K1022100.
Usage and Biology
This is a high throughput sensing module that can sense the presence of AIP-I molecules. This part consists of a pBAD promoter, an AIP sensor infrastructure (BBa_I746101), an AIP inducible promoter P2 and a GFP reporter.
In the natural system, the signalling Auto-inducing peptide<b> (termed AIP) is made from AgrD, while AgrB, a transmembrane protein that is responsible for the cyclization. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1105
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1493
Illegal BamHI site found at 1045
Illegal BamHI site found at 2377 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 295
Illegal AgeI site found at 720 - 1000COMPATIBLE WITH RFC[1000]
Functional Parameters