Difference between revisions of "Part:BBa K4012001"

 
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<partinfo>BBa_K4012001 parameters</partinfo>
 
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===Obtaining the pTEF1 fragment and BsaI digested verification===
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[[Image:T--AISSU_Union--partslast.jpg|thumbnail|500px|center|'''Figure 1:'''
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[https://parts.igem.org/Part:BBa_K4012001] Results of yeast toolkit plasmids enzyme-digested verification]]

Revision as of 10:16, 21 October 2021


pTEF1

A promoter sequence in genome of the Saccharomyces cerevisiae genome, which is used to activate the CHS sequence from Ginkgo biloba in our reconbined plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 714
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 724


Obtaining the pTEF1 fragment and BsaI digested verification

Figure 1: [1] Results of yeast toolkit plasmids enzyme-digested verification