Difference between revisions of "Part:BBa K3806012"

 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3806012 short</partinfo>
 
<partinfo>BBa_K3806012 short</partinfo>
−
 
−
prov
 
−
 
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3806012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3806012 SequenceAndFeatures</partinfo>
 +
 +
===Usage and Biology===
 +
<HTML><a href="https://parts.igem.org/Part:BBa_K3806012" target="_blank"><b>BBa_K3806012</b></a></HTML> contains a codon optimized <i>lacZ</i> gene for optimal expression in <i>E.coli</i>. The codon optimization was performed with the Twist Codon Optimizer Tool. This part is used in <HTML><a href="https://parts.igem.org/Part:BBa_K3806014" target="_blank"><b>BBa_K3806014</b></a></HTML> and <HTML><a href="https://parts.igem.org/Part:BBa_K3806016" target="_blank"><b>BBa_K3806016</b></a></HTML> as reporter gene within a aptazyme-regulated genetic circuit that enables a ligand-dependent colorimetric read-out.
 +
 +
  
  

Revision as of 10:03, 21 October 2021


lacZ reporter gene codon optimized for expression in E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2193
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

BBa_K3806012 contains a codon optimized lacZ gene for optimal expression in E.coli. The codon optimization was performed with the Twist Codon Optimizer Tool. This part is used in BBa_K3806014 and BBa_K3806016 as reporter gene within a aptazyme-regulated genetic circuit that enables a ligand-dependent colorimetric read-out.