Difference between revisions of "Part:BBa K3924052"

 
 
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<partinfo>BBa_K3924052 short</partinfo>
 
<partinfo>BBa_K3924052 short</partinfo>
  
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dCas9-CDA
 
  
 
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<partinfo>BBa_K3924052 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3924052 SequenceAndFeatures</partinfo>
  
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==Profile==
 +
Name: dCas9-CDA<br/>
 +
Base Pairs: 4980bp<br/>
 +
Origin: Escherichia coli <br/>
 +
Properties: A mutanted Cas9 protein fused to a deaminase which can change C into U.<br/>
 +
==Usage and Biology==
 +
dCas9 can bind sgRNA and help CDA to target a specific site of target gene.
 +
==Design and Construction==
 +
For this part, it is a basic consitute of another part.
 +
==Functional Verification==
 +
It has been reported functional ,but we failed to repeat it in our lab due to some reasons explained in Wiki.
 +
==Reference==
 +
Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A. Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018;3(4):423-429. doi:10.1038/s41564-017-0102-6
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 09:22, 21 October 2021


dCas9-CDA


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal BglII site found at 4412
    Illegal BamHI site found at 3378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
  • 1000
    COMPATIBLE WITH RFC[1000]

Profile

Name: dCas9-CDA
Base Pairs: 4980bp
Origin: Escherichia coli
Properties: A mutanted Cas9 protein fused to a deaminase which can change C into U.

Usage and Biology

dCas9 can bind sgRNA and help CDA to target a specific site of target gene.

Design and Construction

For this part, it is a basic consitute of another part.

Functional Verification

It has been reported functional ,but we failed to repeat it in our lab due to some reasons explained in Wiki.

Reference

Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A. Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018;3(4):423-429. doi:10.1038/s41564-017-0102-6