Difference between revisions of "Part:BBa K3788002:Experience"
 
Line 8: Line 8:
 
<h2> PCR amplification of Ghis<i>p20</i> gene : </h2>
 
<h2> PCR amplification of Ghis<i>p20</i> gene : </h2>
  
−
<p> The DNA sequence of Ghis<i>p20</i> was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is <b>570bp </b> and a +/- 600bp band is observed. Amplification of DNAs sequences with primers were validated.</p>
+
<p> The DNA sequence of Ghis<i>p20</i> was amplified by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is <b>570bp </b> and a +/- 600bp band is observed. Amplification of DNAs sequences with primers was validated.</p>
  
 
https://2021.igem.org/wiki/images/a/a3/T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png  
 
https://2021.igem.org/wiki/images/a/a3/T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png  

Latest revision as of 09:08, 21 October 2021


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3788002

PCR amplification of Ghisp20 gene :

The DNA sequence of Ghisp20 was amplified by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 570bp and a +/- 600bp band is observed. Amplification of DNAs sequences with primers was validated.

T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png

Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side).


User Reviews

UNIQb5e6f5313dbe5cd7-partinfo-00000000-QINU UNIQb5e6f5313dbe5cd7-partinfo-00000001-QINU