Difference between revisions of "Part:BBa K3763042"

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==Background==
 
==Background==
 
FadE, which is also called acyl-CoA dehydrogenases , catalyze the first reaction of the b-oxidation cycle. All acyl-CoA dehydrogenases carry noncovalently (but tightly) bound FAD, which is reduced during the oxidation of the fatty acid. As shown in Figure, FADH2 trans- fers its electrons to an electron transfer flavoprotein (ETF). Reduced ETF is reoxidized by a specific oxidoreductase (an iron–sulfur protein), which in turn sends the electrons on to the electron-transport chain at the level of coenzyme Q. The mitochondrial oxidation of FAD in this way eventually results in the net formation of about 1.5 ATPs. The mechanism of the acyl-CoA dehydrogenase involves deprotonation of the fatty acid chain at the a-carbon, followed by hydride transfer from the b-carbon to FAD.<br>
 
FadE, which is also called acyl-CoA dehydrogenases , catalyze the first reaction of the b-oxidation cycle. All acyl-CoA dehydrogenases carry noncovalently (but tightly) bound FAD, which is reduced during the oxidation of the fatty acid. As shown in Figure, FADH2 trans- fers its electrons to an electron transfer flavoprotein (ETF). Reduced ETF is reoxidized by a specific oxidoreductase (an iron–sulfur protein), which in turn sends the electrons on to the electron-transport chain at the level of coenzyme Q. The mitochondrial oxidation of FAD in this way eventually results in the net formation of about 1.5 ATPs. The mechanism of the acyl-CoA dehydrogenase involves deprotonation of the fatty acid chain at the a-carbon, followed by hydride transfer from the b-carbon to FAD.<br>
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[[Image:T--WHU China--42 1 Bacground1.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]]
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                <img style="margin:20px auto 5px auto;" src="https://parts.igem.org/File:T--WHU_China--42_1_Bacground1.png" width="80%">
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                <p style="color:Gray; padding:0px 30px 10px;">Figure1. The FadE catalytic principlei.</p>
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==Design==
 
==Design==

Revision as of 08:53, 21 October 2021

No part name specified with partinfo tag. ( The correct title should be: pFadD promoter with LacI repressor regulating downstream RFP)

Background

FadE, which is also called acyl-CoA dehydrogenases , catalyze the first reaction of the b-oxidation cycle. All acyl-CoA dehydrogenases carry noncovalently (but tightly) bound FAD, which is reduced during the oxidation of the fatty acid. As shown in Figure, FADH2 trans- fers its electrons to an electron transfer flavoprotein (ETF). Reduced ETF is reoxidized by a specific oxidoreductase (an iron–sulfur protein), which in turn sends the electrons on to the electron-transport chain at the level of coenzyme Q. The mitochondrial oxidation of FAD in this way eventually results in the net formation of about 1.5 ATPs. The mechanism of the acyl-CoA dehydrogenase involves deprotonation of the fatty acid chain at the a-carbon, followed by hydride transfer from the b-carbon to FAD.

Figure 1. Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression. Induced at time=0h.

Design

In our experiment, we hope to improve the β-oxidation capacity of our engineered bacteria by overexpressing FadE protein . As shown in the figure below, we constructed a recombinant plasmid containing FadE gene and introduced it into our engineered bacteria.

Figure2.Schematic diagram of recombinant vector containing FadE.

Result

After confirming that we correctly constructed and transferred the recombinant plasmid into the engineering strain E. coli DH5 α, we used arabinose to induce the expression of FadE and tested its improvement on the fatty acid decomposition ability of engineered bacteria. Our experimental results showed that induced overexpression of FadE did not significantly improve the fatty acid decomposition ability of engineered bacteria, and did not reproduce the experimental results in references.

Figure1. Changes of fatty acid decomposition ability of engineering bacteria overexpressing FadE protein.

In order to explore the reasons for the failure of the experiment, we detected the protein expression more carefully. After inducing the expression of FadE proteins, they were purified by Ni2+ affinity chromatography column. After purification, SDS-PAGE results showed that the molecular weight of our target band was about 20 kDa lower than our predicted value. It is speculated that the engineering strain we used is E. coli DH5 α. The endogenous protease system of this strain has not been artificially knocked out, so the overexpressed protein is easy to be degraded. Considering this possibility, we replaced our engineered strain with E. coli BL21 strain. Then we purified the protein by Ni2+ affinity chromatography column and detected it by SDS-PAGE. The results showed that the bands of FadD and FadE protein were in line with our prediction.


Sequence and Features No part name specified with partinfo tag.