Difference between revisions of "Part:BBa K648007"

 
 
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This is the mid-sized linker of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the six amino acids GGSGGS and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.  
 
This is the mid-sized linker of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the six amino acids GGSGGS and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.  
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This linker was cited in a very interesting article that talks about its high potential for use in "usefulness of tags and linker in plant molecular pharming", which can also be used in recombinant proteins. It was also cited in the American patent application for CAS9 FUSION MOLECULES, GENE EDITING SYSTEMS AND METHODS OF USING THEM (Request # 20190136210), as an additional linker, being a 6AA Medium Fusion Protein Linker. <br>
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Isoelectric Point: 5,52 <br>
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Molecular Weight: 420,38Da <br>
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<partinfo>BBa_K648007 parameters</partinfo>
 
<partinfo>BBa_K648007 parameters</partinfo>
 
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===REFERENCES===
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#Makhzoum, Abdullah & Tahir, Shifa & Locke, Majorie & Trémouillaux-Guiller, Jocelyne & Hefferon, Kathleen. (2014). An in silico overview on the usefulness of tags and linkers in plant molecular pharming. Plant Science Today. 1. 201-212. 10.14719/pst.2014.1.4.72.

Latest revision as of 08:34, 21 October 2021


Medium 6AA Fusion Protein Linker: GGSGGS with Standard 25 Prefix/Suffix

This is the mid-sized linker of the three fusion protein linkers created by the Penn State 2011 iGEM team. It encodes for the six amino acids GGSGGS and has a prefix and suffix compatible with assembly standard 25. It is used in one of the variants of our Fast-Fusion protein reporter system.

This linker was cited in a very interesting article that talks about its high potential for use in "usefulness of tags and linker in plant molecular pharming", which can also be used in recombinant proteins. It was also cited in the American patent application for CAS9 FUSION MOLECULES, GENE EDITING SYSTEMS AND METHODS OF USING THEM (Request # 20190136210), as an additional linker, being a 6AA Medium Fusion Protein Linker.

Isoelectric Point: 5,52

Molecular Weight: 420,38Da



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


REFERENCES

  1. Makhzoum, Abdullah & Tahir, Shifa & Locke, Majorie & Trémouillaux-Guiller, Jocelyne & Hefferon, Kathleen. (2014). An in silico overview on the usefulness of tags and linkers in plant molecular pharming. Plant Science Today. 1. 201-212. 10.14719/pst.2014.1.4.72.