Difference between revisions of "Part:BBa K3895016"

Revision as of 08:29, 21 October 2021

Optimized keratinase KerA

Introduction

This composite part is an optimized version of KERA that can be constructed into pSB1C3 plasmid with the highest protein expression in E.coli BL21(DE3). The KERA (BBa_K1717171) is a basic part comprised from a synthesized KERA protein coding sequence. The gene can be expressed in cells by looking for clear areas of cell growth on AGAR plates of skimmed milk, and by observing keratin-containing substrates (feathers, hair, chemosynthetic keratin, etc.) growing with cells.

Plasmid Construction

Experiment details

• Throughput: 2500
• Organism: E. coli • Strain type: BL21(DE3) • Temperature: 37°C • Media: 250 ml LB • Period of time: 16 hours • Plasmid backbone: pSB1C3

Sequence and Features