Difference between revisions of "Part:BBa K3712022"

 
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3712022 short</partinfo>
 
<partinfo>BBa_K3712022 short</partinfo>
 +
===USAGE AND BIOLOGY===
 +
<p>We connected the TLR2 antagonist to 27ELP in order to verify the ability of the TLR2 antagonist to bind to acylated lipoproteins and the heat-responsive aggregation effect of ELP after TLR2 and ELP were connected.</p>
  
−
We connected the TLR2 antagonist to 27ELP in order to verify the ability of the TLR2 antagonist to bind to acylated lipoproteins and the heat-responsive aggregation effect of ELP after TLR2 and ELP were connected.
+
===RESULTS===
  
−
<!-- Add more about the biology of this part here
+
<!-- Add more about the biology of this part here-->
−
===Usage and Biology===
+
<html>
 +
<head>
 +
<meta charset="utf-8">
 +
<title>Part:BBa_K3712022</title>
 +
</head>
 +
<body>
 +
<center><img src="https://2021.igem.org/wiki/images/5/5f/T--HUST2-China--SDS-PAGE_of_Wild-type_TLR2-27ELP%2C_Optimized_TLR2-27_ELP_expression_results.png" style="width:793px;height:360px"></center>
 +
 
 +
<p><b>Figure 1.</b>SDS-PAGE of Wild-type TLR2-27ELP, Optimized TLR2-27 ELP expression results. Near the 25kDa marker, the binds of Wild-type TLR2-27ELP and Optimized TLR2-27 ELP after induction are darker than the expression system without induction.</p>
 +
 
 +
<h1>Future direction</h1>
 +
 
 +
<p>Unfortunately, due to time issues, we have no way to complete the purification of TLR2 antagonist. In the future, we will try different linkers to connect Wild-type/Optimized TLR2 antagonist and 27ELP.</p>
 +
 
 +
<br>
 +
</body>
 +
</html>
  
 
<!-- -->
 
<!-- -->

Revision as of 08:19, 21 October 2021


Wild-type TLR2 antagonist-27 ELP

USAGE AND BIOLOGY

We connected the TLR2 antagonist to 27ELP in order to verify the ability of the TLR2 antagonist to bind to acylated lipoproteins and the heat-responsive aggregation effect of ELP after TLR2 and ELP were connected.

RESULTS

Part:BBa_K3712022

Figure 1.SDS-PAGE of Wild-type TLR2-27ELP, Optimized TLR2-27 ELP expression results. Near the 25kDa marker, the binds of Wild-type TLR2-27ELP and Optimized TLR2-27 ELP after induction are darker than the expression system without induction.

Future direction

Unfortunately, due to time issues, we have no way to complete the purification of TLR2 antagonist. In the future, we will try different linkers to connect Wild-type/Optimized TLR2 antagonist and 27ELP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 178