Difference between revisions of "Part:BBa K4044004"
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This is a composite part of BphP1 construction under pj23119 promoter (BBa_J23119) fused to QPAS1-Gal4 construction forming a whole operon; with YFP gene under p119_G4 promoter (BBa_K4044010). The part is assembled for testing the accuracy and dynamic range of the system with p119_G4 promoter. | This is a composite part of BphP1 construction under pj23119 promoter (BBa_J23119) fused to QPAS1-Gal4 construction forming a whole operon; with YFP gene under p119_G4 promoter (BBa_K4044010). The part is assembled for testing the accuracy and dynamic range of the system with p119_G4 promoter. | ||
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Latest revision as of 08:14, 21 October 2021
p119_BphP1_QPAS1-Gal4 test system with p119_G4-YFP
This is a composite part of BphP1 construction under pj23119 promoter (BBa_J23119) fused to QPAS1-Gal4 construction forming a whole operon; with YFP gene under p119_G4 promoter (BBa_K4044010). The part is assembled for testing the accuracy and dynamic range of the system with p119_G4 promoter.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 3222
Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 301
Illegal BamHI site found at 1876
Illegal BamHI site found at 2969 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 815
Illegal PstI site found at 1112
Illegal PstI site found at 1559
Illegal NgoMIV site found at 457
Illegal NgoMIV site found at 1208
Illegal NgoMIV site found at 1705 - 1000COMPATIBLE WITH RFC[1000]