Difference between revisions of "Part:BBa K3939998"
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This part is constructed by two LoxP sequences and a reversed pGal promoter. the pGal promoter is not a tightly regulated promoter. thus,we used estrogen and galactose to induce Cas9 expression, allowing this element to be regulated more precisely. | This part is constructed by two LoxP sequences and a reversed pGal promoter. the pGal promoter is not a tightly regulated promoter. thus,we used estrogen and galactose to induce Cas9 expression, allowing this element to be regulated more precisely. | ||
| − | In our project, we use it to cut chromosomes to make chromosome-free eukaryotic cell-- <b>CREATE</b>. More details about this part, please click [https://2021.igem.org/Team:Tianjin/Measurement Measurement] | + | In our project, we use it to cut chromosomes to make chromosome-free eukaryotic cell-- <b>CREATE</b>. More details about this part, please click [https://2021.igem.org/Team:Tianjin/Measurement Measurement]. |
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Revision as of 08:09, 21 October 2021
Cas9 with flipped promoter
Brief introduction
This part is constructed by two LoxP sequences and a reversed pGal promoter. the pGal promoter is not a tightly regulated promoter. thus,we used estrogen and galactose to induce Cas9 expression, allowing this element to be regulated more precisely. In our project, we use it to cut chromosomes to make chromosome-free eukaryotic cell-- CREATE. More details about this part, please click Measurement. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 749
Illegal BglII site found at 1686
Illegal BamHI site found at 2504
Illegal XhoI site found at 4042 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3238
Illegal AgeI site found at 402 - 1000COMPATIBLE WITH RFC[1000]