Difference between revisions of "Part:BBa K3939998"
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===Usage and Biology===
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===Characterization===
 
<b> Use fluorescent microscope to test the function of part</b>
 
<b> Use fluorescent microscope to test the function of part</b>
 
1.Experimental principle & purpose：
 
1.Experimental principle & purpose：

Revision as of 08:00, 21 October 2021


Cas9 with flipped promoter

Brief introduction

This part is constructed by two LoxP sequences and a reversed pGal promoter. the pGal promoter is not a tightly regulated promoter. thus,we used estrogen and galactose to induce Cas9 expression, allowing this element to be regulated more precisely. In our project, we use it to cut chromosomes to make chromosome-free eukaryotic cell-- CREATE. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 749
    Illegal BglII site found at 1686
    Illegal BamHI site found at 2504
    Illegal XhoI site found at 4042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3238
    Illegal AgeI site found at 402
  • 1000
    COMPATIBLE WITH RFC[1000]