Difference between revisions of "Part:BBa K4083010"

 
 
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<partinfo>BBa_K4083010 short</partinfo>
 
<partinfo>BBa_K4083010 short</partinfo>
  
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czsczc
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This gene encodes the repressor of the lactose operon.
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===Usage and Biology===
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This gene is universally used to control the plac or its derivatives as ptac promoters [1]. However,
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For our main project goal, we reversed the lacI gene according to the pRGPDuo2 plasmid.
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===Reference===
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[1]Swerdlow, S. J., & Schaaper, R. M. (2014). Mutagenesis in the lacI gene target of E. coli: Improved analysis for lacId and lacO mutants. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 770, 79–84. https://doi.org/10.1016/j.mrfmmm.2014.09.004
  
 
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Latest revision as of 07:58, 21 October 2021


LacI Reverse

This gene encodes the repressor of the lactose operon.

Usage and Biology

This gene is universally used to control the plac or its derivatives as ptac promoters [1]. However, For our main project goal, we reversed the lacI gene according to the pRGPDuo2 plasmid.

Reference

[1]Swerdlow, S. J., & Schaaper, R. M. (2014). Mutagenesis in the lacI gene target of E. coli: Improved analysis for lacId and lacO mutants. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 770, 79–84. https://doi.org/10.1016/j.mrfmmm.2014.09.004

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]